Carbon-X vs Qwen3-4B-Instruct-2507

Carbon-X

A DNA specialist cousin that another agent calls for variant-effect and sequence questions, with DNA kept on the machine. Hugging Face's Q4_K_M GGUF needs about 12 GB and Q8_0 about 19 GB, on llama.cpp b11742 or later. It isn't clinically validated, it isn't trained for tool calls or reasoning, and the card contradicts its own GGUF repository on llama.cpp support.

Qwen3-4B-Instruct-2507

A small, fast cousin for instruction following, short tool-calling jobs and long documents on modest hardware, with no thinking step. It runs in about 3 GB at 4 bits on every common runtime, and two providers host it. The main risk is neglect, with no file changes in over a year, no answers from Alibaba and no safety results.

Carbon-X

1. Quickest start is llama-server -hf HuggingFaceBio/Carbon-X-GGUF:Q8_0 -ngl 99 -c 40960 --jinja --chat-template-kwargs '{"enable_thinking": false}' --temp 0.6 --top-p 0.95 --top-k 20 --min-p 0, on llama.cpp b11742 or later
2. With vLLM or Transformers pass trust_remote_code, and expect the tokenizer to fetch Qwen/Qwen3-4B-Base at a pinned revision on first load
3. Keep enable_thinking false and the card's sampling (temperature 0.6, top_p 0.95, top_k 20), the settings every reported result used
4. Keep DNA in files and pass sequences by name, compute positions and base changes in code and send them as context, the card says the model miscounts long sequences
5. Only A, C, G and T form 6-mers, so filter N and other IUPAC codes first or they become a single oov token
6. Strip the dna tags from answers if you need bare sequence, llama.cpp keeps them in the text
7. Cap max_tokens for next-base questions, long DNA generation is slow and degrades

Qwen3-4B-Instruct-2507

1. Quickest start is ollama run qwen3:4b-instruct, 2.5 GB. Plain qwen3:4b is the Thinking-2507 edition, not this one
2. On our reference machine use BF16 with vllm serve Qwen/Qwen3-4B-Instruct-2507 --max-model-len 262144 --enable-auto-tool-choice --tool-call-parser hermes
3. Use the hermes parser in vLLM or qwen25 in SGLang, the template emits JSON inside tool_call tags
4. Don't pass enable_thinking, this edition never thinks and the card says the flag isn't needed
5. Sampling per the card is temperature 0.7, top_p 0.8, top_k 20 and min_p 0, with presence_penalty up to 2 against repetition
6. Drop the context to 32,768 tokens if memory runs out, as the card suggests
7. Validate JSON yourself or use the runtime's guided decoding, since structured output isn't documented