Carbon-X vs Gemma 4 31B IT

Carbon-X

A DNA specialist cousin that another agent calls for variant-effect and sequence questions, with DNA kept on the machine. Hugging Face's Q4_K_M GGUF needs about 12 GB and Q8_0 about 19 GB, on llama.cpp b11742 or later. It isn't clinically validated, it isn't trained for tool calls or reasoning, and the card contradicts its own GGUF repository on llama.cpp support.

Gemma 4 31B IT

A dense multimodal cousin with documented tool calling, optional thinking and a 256K context, for general agent work and image reading on one 24 GB GPU through Google's 4-bit build. It runs in every major runtime under plain Apache 2.0. The main risk is that Google publishes no safety results and has gone quiet on the repository since July.

Carbon-X

1. Quickest start is llama-server -hf HuggingFaceBio/Carbon-X-GGUF:Q8_0 -ngl 99 -c 40960 --jinja --chat-template-kwargs '{"enable_thinking": false}' --temp 0.6 --top-p 0.95 --top-k 20 --min-p 0, on llama.cpp b11742 or later
2. With vLLM or Transformers pass trust_remote_code, and expect the tokenizer to fetch Qwen/Qwen3-4B-Base at a pinned revision on first load
3. Keep enable_thinking false and the card's sampling (temperature 0.6, top_p 0.95, top_k 20), the settings every reported result used
4. Keep DNA in files and pass sequences by name, compute positions and base changes in code and send them as context, the card says the model miscounts long sequences
5. Only A, C, G and T form 6-mers, so filter N and other IUPAC codes first or they become a single oov token
6. Strip the dna tags from answers if you need bare sequence, llama.cpp keeps them in the text
7. Cap max_tokens for next-base questions, long DNA generation is slow and degrades

Gemma 4 31B IT

1. On the reference machine run llama-server -hf google/gemma-4-31B-it-qat-q4_0-gguf --hf-file gemma-4-31B_q4_0-it.gguf --jinja -c 32768, adding gemma-4-31B-it-mmproj.gguf for images
2. Or ollama run gemma4:31b for the 20 GB Q4_K_M build
3. In vLLM serve with --enable-auto-tool-choice --tool-call-parser gemma4 --reasoning-parser gemma4, as vLLM's recipe does
4. Thinking is off by default, so pass enable_thinking: true in chat_template_kwargs to turn it on, and expect the reasoning in the thought channel block
5. Drop earlier thoughts from the history except on tool-call turns, as the card asks
6. Use the card's sampling, temperature 1.0, top_p 0.95, top_k 64
7. Validate function names and arguments before running a call, and check JSON output yourself or use the runtime's guided decoding
8. The w4a16 build relies on Marlin INT4 kernels that vLLM marks unsupported on AMD, so use the GGUF on the reference machine